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Detection and first characterization of a cell‐wall lytic activity in Chlorella ellipsoidea C‐27
Author(s) -
Satoh Hitoshi,
Takeda Hiroshi
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb05972.x
Subject(s) - cell wall , lytic cycle , polysaccharide , glycosidic bond , chlorella , biochemistry , chemistry , protease , hydrolysis , xylan , enzyme , biology , algae , botany , virus , virology
Cell wall lytic activity was detected in Chlorella ellipsoidea Gernick IAM C‐27 using the [ 14 C]‐labeled cell wall as substrate. The highest activity was obtained at pH 8. and the solubilized product was a polysaccharide of high molecular weight. The lytic activity appeared to be a protease and did not hydrolyse glycosidic bonds of the cell wall polysaccharides. The activity probably solubilizes the cell wall by cleaving the peptide bonds that interconnect the polysaccharides of the cell wall.