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Directed mutagenesis to probe the structure and function of photosystem II
Author(s) -
Carpenter Shelly D.,
Vermaas Wim F. J.
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb05665.x
Subject(s) - mutagenesis , mutant , photosystem ii , photosynthetic reaction centre , site directed mutagenesis , homology modeling , function (biology) , chemistry , biophysics , computational biology , structure function , biology , biochemistry , microbiology and biotechnology , photosynthesis , physics , gene , particle physics , enzyme
In the last few years various advances have contributed to an increased understanding of Photosystem II (PS II). Most notably, the X‐ray diffraction analysis of crystallized bacterial reaction centers, along with the recognition that there is functional and structural homology between the bacterial reaction center and PS II, has led to detailed information regarding the potential function of individual proteins and residues in the PS II complex. In‐depth studies of PS II structure and function, however, require the availability of specific mutants in which certain proteins have been altered. Recombinant DNA technology has provided the methodology by which generation of such mutants has become feasible. This minireview focuses on methods for mutagenesis of PS II components and on the impact of mutant analysis on the understanding of PS II structure and function.