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Etiolation as a pretreatment for in vitro establishment and multiplication of mature chestnut
Author(s) -
Ballester A.,
Sánchez M. C.,
Vieitez A. M.
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb05659.x
Subject(s) - etiolation , shoot , explant culture , multiplication (music) , biology , botany , in vitro , horticulture , micropropagation , mathematics , biochemistry , combinatorics , enzyme
Localized etiolation of branches in the crown of a 30‐year‐old chestnut tree produced plant material that responded much better to establishment and multiplication in vitro than unetiolated material, whose cultures were very difficult to maintain (response being measured in terms of the percentage of cultures established, the mean number of shoots formed per explant, the number of 8‐mm segments per new shoot, the length of longest shoot in each culture, and the coefficient of multiplication). Only 22% of the initial explants from unetiolated material were successfully established, as against 79% for etiolated material, with similar differences between the coefficients of multiplication of the two lines in successive cultures. Accordingly, partial etiolation of branches is proposed as a suitable pretreatment for in vitro propagation of selected mature trees, when physiologically juvenile materials such as stump sprouts, epicormic shoots or root suckers are not available.