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Internode length in Pisum . Estimation of GA 1 levels in genotypes Le, le and le d
Author(s) -
Ross John J.,
Reid James B.,
Gaskin Paul,
MacMillan Jake
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb05627.x
Subject(s) - pisum , sativum , genotype , biology , isotope dilution , elongation , locus (genetics) , microbiology and biotechnology , horticulture , chemistry , biochemistry , chromatography , gene , mass spectrometry , materials science , ultimate tensile strength , metallurgy
The levels of GA 1 , 3‐epiGA 1 and GA 8 in genotypes Le, le and le d of Pisum sativum L. were determined by gas chromatography‐selected ion monitoring (GC‐SIM) after feeds of [ 3 H, 13 C]‐GA 20 to each genotype. The levels of endogenous and [ 13 C]‐labelled metabolites were determined by reverse isotope dilution with unlabelled GA 1 , 3‐epiGA 1 and GA 8 . The results demonstrate a quantitative relationship between the level of GA 1 and the extent of elongation both on a per plant and a per g fresh weight basis. These results are consistent with previous findings in peas and other species possessing a predominant early 13‐hydroxylation pathway for GA biosynthesis. The levels of 3‐epiGA 1 also decreased in the genotypic sequence Le, le, le d although not as rapidly as for the level of GA 1 . This may suggest that the alleles at the le locus also influence the formation of 3‐epiGA 1 .