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Bivariate cytofluorimetric analysis of nuclear protein and DNA relative to cell kinetics during germination of Pisum sativum seed
Author(s) -
Sgorbati Sergio,
Sparvoli Elio,
Levi Marisa,
Chiatante Donato
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb05612.x
Subject(s) - meristem , sativum , pisum , nuclear dna , biology , population , cell cycle , germination , microbiology and biotechnology , interphase , botany , cell , biochemistry , shoot , demography , sociology , mitochondrial dna , gene
By means of a biparametric cytofluorimetric analysis it is possible to distribute meristematic plant cells in a variety of cell cycle sub‐compartments, unidentifiable by DNA measurements alone. In this work, an asynchronous proliferating cell population of pea root meristems was divided into different sub‐compartments of the cell cycle phases, i.e. G1A, G1B, S. G2A and G2B on the basis of their DNA‐nuclear protein content. By means of the same biparametric analysis, differentiated mesophyll cells and quiescent cells of embryo roots, indicated as G0 and G2Q, were distinguished from cycling cells by their low nuclear protein content. These results conform to those of some analyses performed on animal cells in culture and show that it is possible to get a major insight into cell cycle kinetics and its control in a natural system such as root meristem.