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Two‐dimensional protein profiles of palisade parenchyma and of spongy parenchyma of Vicia faba leaf
Author(s) -
Shen Liming,
Outlaw William H.
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb05397.x
Subject(s) - parenchyma , vicia faba , staining , biology , stain , homogeneous , botany , physics , genetics , thermodynamics
Specific probes such as targeted‐enzyme assays have failed to reveal qualitative differences between the metabolism of spongy parenchyma and that of palisade parenchyma cells. To determine whether the results from these definite assays support a general conclusion concerning the overall biochemical similarity of these cells, two‐dimensional protein profiles have been constructed for these two types of cells. Protein extracts were prepared from freeze‐dried homogeneous tissue samples or from isolated protoplasts of Vicia faba L. cv. Longpod leaves. Proteins separated by micro two‐dimensional gel electrophoresis were stained with silver. The relative apparent abundance (based on visual judgment of the area and intensity of staining) was estimated for each spot. Pairwise comparison of nominally 125 stained proteins of each of approximately 75 gel pairs did not reveal a consistent qualitative difference between the profiles. Recognizing that polypeptides escaped detection in our investigation through loss, failure to stain sufficiently, or incomplete resolution, we qualify our conclusion that spongy parenchyma and palisade are similar in their overall protein complement.