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Kinetics of efflux of K + from cultured rose cells treated with ultraviolet light
Author(s) -
Huerta Alfredo J.,
Murphy Terence M.
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb05387.x
Subject(s) - efflux , kinetics , rosa × damascena , extracellular , chemistry , membrane potential , biophysics , rose bengal , chromatography , biochemistry , biology , physics , organic chemistry , quantum mechanics , essential oil
Irradiation of cultured rose ( Rosa damascena Mill. cv. Gloire de Guilan) cells with ultraviolet light caused of loss of K + , which occurred with sigmoid kinetics. The kinetics of loss of K + were not changed when the extracellular concentration of K + was held constant during the period of efflux. Furthermore, the rate of loss of K + was approximately the same even though the K + concentration in the medium was increased from 0.1 to 10 m M . The kinetics of uptake of the lipophilic methyltriphenylphosphonium cation, an indicator of the plasma membrane potential, were linear throughout the period of K + efflux, suggesting that the starting and stopping of K + efflux do not reflect a passive response to changes in the membrane potential of the cells. The results are interpreted in terms of activation and inactivation of an efflux channel or pump for K + .