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An exo‐β‐ d ‐glucanase derived from Zea coleoptile walls with a capacity to elicit cell elongation
Author(s) -
Labrador Emilia,
Nevins Donald J.
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb05380.x
Subject(s) - coleoptile , chromatofocusing , glucanase , elongation , cell wall , size exclusion chromatography , xyloglucan , glucan , chemistry , hydrolysis , zea mays , biochemistry , chromatography , enzyme , biology , materials science , agronomy , metallurgy , ultimate tensile strength
Cell wall proteins were extracted from maize coleoptiles, Zea mays L. B37 x MO 17, with high concentrations of LiCl. Ion‐exchange, chromatofocusing and gel‐filtration chromatography were employed extensively to purify exo‐β‐glucanase activity from the extract. The purified enzyme functioned as an exo‐(1→3)‐β‐glucanase (E.C. 3.2.1.58) and as a glucosidase (E.C. 3.2.1.21) capable of extensive hydrolysis of the native Zea wall (1→3), (1→4)‐β‐ d ‐glucan, yielding glucose as the final product. The exoglucanase also enhances elongation of maize coleoptile sections in both the presence and absence of exogenous IAA.