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Detection and identification of gibberellins in Sitka spruce ( Picea sitchensis ) of different ages and coning ability by bioassay, radioimmunoassay and gas chromatography – mass spectrometry
Author(s) -
Moritz Thomas,
Philipson J. Julian,
Odén Per Christer
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb04634.x
Subject(s) - gibberellin , chromatography , chemistry , gas chromatography , mass spectrometry , gas chromatography–mass spectrometry , high performance liquid chromatography , bioassay , radioimmunoassay , enzymatic hydrolysis , hydrolysis , botany , biology , biochemistry , genetics
Gibberellins Al (GA 1 ), GA 3 , GA 4 , GA 9 , and after enzymatic hydrolysis of GA‐conjugate‐like fractions, GA 9 and GA 15 , were identified in shoots of Sitka spruce [ Picea sitchensis (Bong.) Carr.] of different ages by combined gas chromatography‐mass spectrometry (GC‐MS). The purification and separation of the GAs involved the use of reverse phase and normal phase high performance liquid chromatography (HPLC). The Tan‐ginbozu dwarf rice bioassay and binding to antibodies raised against GA 1 , GA 4 and GA 9 were used for detection of GA‐like substances. The qualitative differences between the three ages of plant material were the presence of GA 3 and GA 1 in the 48‐year‐old material and the absence of detectable amounts of GA 4 in the same material. This indicates a difference in GA metabolism which may reflect the difference in ability to form reproductive buds.