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Measurement of light within thin plant tissues with fiber optic microprobes
Author(s) -
Vogelmann Thomas C.,
Knapp Alan K.,
McClean Therese M.,
Smith William K.
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb09173.x
Subject(s) - microprobe , cucurbita pepo , collimated light , optical fiber , thin layer , fiber , light scattering , optics , materials science , etiolation , palisade cell , chemistry , layer (electronics) , scattering , botany , mineralogy , laser , biology , physics , composite material , biochemistry , enzyme
Vogelmann, T. C., Knapp, A. K., McClean, T. M. and Smith, W. K. 1988. Measurement of light within thin plant tissues with fiber optic microprobes. ‐ Physiol. Plant. 72: 623–630. The measurement of light with fiber optic microprobes has been extended to thin (200–300 μm) plant tissue samples. To test the method, light measurements were made in thin aqueous films and paradermal sections from 10‐day‐old etiolated Cucurbita pepo L. cv. Fordhook cotyledons. The measurements obtained were highly reproducible. Paradermal sections of spongy mesophyll that were irradiated with collimated light scattered light more effectively than the palisade layer of intact cotyledons. These results demonstrate that different plant tissues have different light scattering characteristics. The successful extension of the fiber optic microprobe technique to thin systems makes it possible to examine the optical properties of different cell layers within leaves and other plant organs.