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Somatic embryogenesis and plant regeneration from suspension cultures of Picea glauca (White spruce)
Author(s) -
Hakman Inger,
Arnold Sara
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb09168.x
Subject(s) - subculture (biology) , somatic embryogenesis , abscisic acid , biology , botany , cytokinin , auxin , gibberellic acid , tissue culture , regeneration (biology) , murashige and skoog medium , embryo , embryogenesis , microbiology and biotechnology , germination , biochemistry , in vitro , gene
Hakman, I. and von Arnold, S. 1988. Somatic embryogenesis and plant regeneration from suspension cultures of Picea glauca (White spruce). ‐ Physiol. Plant. 72: 579–587. Plantlets were regenerated from long‐term embryogenic cultures of Picea glauca (Moench) Voss. (White spruce). Embryogenic calli, initiated from immature zygotic embryos and maintained by monthly subculture for 16 months, were used to establish suspension cultures. Small somatic embryos were continuously produced in liquid culture medium containing auxin and cytokinin and the cultures showed a sustained regeneration capacity for >6 months. Somatic embryos propagated in the suspension cultures developed further into embryos bearing cotyledons, about 1 month after transfer to solidified medium containing abscisic acid. Electron microscopic examination revealed that storage nutrients, lipids, proteins and carbohydrates, accumulated in the somatic embryos during this treatment with abscisic acid (ABA). Upon subculture to medium lacking plant growth regulators such embryos could develop into small green plantlets.