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Establishment, graft union characteristics and growth of Prunus micrografts
Author(s) -
Gebhardt K.,
Goldbach H.
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb06637.x
Subject(s) - rootstock , shoot , prunus , meristem , horticulture , biology , prunus cerasus , cultivar , botany , elongation , callus , sour cherry , materials science , ultimate tensile strength , metallurgy
A micrografting technique for use on shoots derived by shoot‐tip culture is described. Autografts of Prunus domestica cv. Hauszwetsche as well as heterografts of several sour cherry cultivars ( Prunus cerasus L. cvs Schattenmorelle, Weiroot 158, Köröser) were established. Successful graft formation in vitro was confirmed by translation of 86 Rb + from the stock root into growing scion tissues. A mechanically strong graft union was formed during the course of a 3‐week subculture of micrografts in a liquid medium without the addition of growth regulators. In the case of graft rejection, 86 Rb + was mainly attracted to new developing shoots from lateral meristems of the stock plant. Histological examination of the graft union revealed callus formation, cytodifferentiation and xylogenesis leading to the formation of vascular connections. Stem elongation after micrografting was related to vigour of the stock and scion genotypes. Early stem elongation could be used as a criterion for preselection of growth vigour in graft combinations. Micrografts were transferred to soil and grown in the greenhouse.