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Changes in the nucleotide pools in leaves and buds of tobacco plants upon treatment with 2,4‐dichlorophenoxyacetic acid
Author(s) -
Golebski Waldemar,
Meyer Reiner,
Wagner Karl G.
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb06618.x
Subject(s) - nicotiana tabacum , 2,4 dichlorophenoxyacetic acid , auxin , nucleotide , energy charge , nad+ kinase , biology , solanaceae , botany , biochemistry , ribonucleotide , chemistry , horticulture , enzyme , gene , adenylate kinase
Tobacco ( Nicotiana tabacum L. cv. Samsun) plants were treated once with 2,4‐dichlorophenoxyacetic acid (2,4‐D) at the 8‐leaf stage. The effect of the herbicide on leaf metabolism was followed over 7 days by determination of the ribonucleotide pools, including NAD + , NADP + and UDP‐sugars, by high‐preformance liquid chromatography. 2,4‐D treatment resulted in large changes in the nucleotide concentrations, the magnitude and sign of which were dependent upon the leafage. The nucleotide pools decreased in the apical tissue, but increased strongly in the mature leaves with the highest relative increase in the oldest leaf tested. The time course of the changes revealed a maximum on day 5 after 2,4‐D treatment. The increase in the adenine nucleotide pools, energy charge and the NADVNADP + ratio are interpreted to indicate a stress situation. The different responses of young, mature and senescent tissue to the synthetic auxin could reflect their different inherent sensitivity due to the natural auxin gradient.