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Nucleotide status and protein synthesis in vivo in the apices of juvenile and mature Sequoiadendron giganteum during budbreak
Author(s) -
Bon MarieClaude
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb06381.x
Subject(s) - gtp' , clone (java method) , in vivo , methionine , guanine , biology , juvenile , nucleotide , biochemistry , protein biosynthesis , botany , enzyme , genetics , amino acid , dna , gene
Adenine and guanine nucleotide contents of isolated apices collected from a juvenile and a mature clone of Sequoiadendron giganteum (Lindl.) Buchholz during budbreak were determined. GDP and GTP contents were significantly higher in the juvenile clone apex than in the mature ones, whereas there was no difference in ATP concentration between the two materials. In vivo, induction of protein synthesis was similar in the two clones after 10 min of [ 35 S]‐methionine labeling. The increase of [ 35 S]‐methionine‐tRNAs and labeled proteins continued up to 30 min for the juvenile clone. They markedly declined for the mature clone after 10 min. Only the diminution of this in vivo protein synthesis was well correlated with a decrease in GTP content.