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Growth inhibitors in oat grains. II. Bioassays for characterization of a new substance in leachate of oat hulls ( Avena sativa ) regulating root growth
Author(s) -
Häggquist M.L.,
Widell K.O.,
Fredriksson M.,
Liljenberg C.
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb05854.x
Subject(s) - avena , coleoptile , seedling , thistle , auxin , endosperm , leachate , botany , biology , ethylene , chemistry , poaceae , horticulture , agronomy , biochemistry , environmental chemistry , gene , catalysis
Oat hull leachate, auxin and ethylene cause similar effects on oat ( Avena sativa L. cv. Risto) seedlings: inhibited root elongation, swollen roots and extensive root hair formation. Micrographs reveal that the leachate caused a reorientation of the cell expansion in the root from a longitudinal to a radial direction. Dry weights showed that the distribution of nutrients from the endosperm into the seedling was not inhibited, but regulated by the leachate. Tests with Ag + , inhibiting the effects of ethylene, as well as biotests with seeds of Amaranthus caudatus L. cv Mörk Purpur excluded ethylene from being responsible for the morphological changes. Results of growth tests and Avena coleoptile bioassuys indicated that oat hull leachate contains a substance that is not identical with IAA, but mediates the effects of endogenous IAA of the seedling. In a parallell investigation (Häggqquist, M.‐L. et al. 1988. ‐ Physiol. Plant. 72: 423–127) the active substance of an high performance liquid chromatography (HPLC) fraction of the leachate was identified as tryptophan.