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Respiration, cyanide‐insensitive oxygen uptake and oxidative phosphorylation in cyanobacteria
Author(s) -
Scherer Siegfried,
Häfele Ursula,
Krüger Gert H. J.,
Böger Peter
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb05848.x
Subject(s) - cyanide , oxidative phosphorylation , anabaena variabilis , cyanobacteria , oxygen , respiration , biochemistry , chemistry , electron transport chain , cytochrome , biology , bacteria , botany , inorganic chemistry , enzyme , organic chemistry , genetics
Cyanide‐insensitive oxygen uptake in the dark of 9 species of cyanobacteria was 6–20% of the total oxygen uptake of intact cells. In Phormidium , no cyanideinsensitive oxygen uptake was observed. In intact cells, the I 50 value for cyanide was significantly lower in cyanobacteria of the taxonomic sections I to III (1–9 μ M ) than in those from section IV and V (10–60 μ M ). Cyanide‐insensitive oxygen uptake in the cell‐free system of Anabaena variabilis was not affected by typical inhibitors of the alternative pathway of plants. Cell‐free oxidation of cytochrome c was completely inhibited by cyanide with an I 50 value of 0.5–1 μ M . Electron transport of intact cells without cyanide present yielded P/O ratios of 0.7–3.0. The data on oxidative phosphorylation using intact cells and the cell‐free system, indicate that cyanide‐insensitive oxygen uptake is not coupled to ATP formation.