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The marginal regions of thylakoid membranes: A partial characterization by polyoxyethylene sorbitan monolaurate (Tween 20) solubilization of spinach thylakoids
Author(s) -
Webber Andrew N.,
PlattAloia Kathryn A.,
Heath Robert L.,
Thomson William W.
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb05835.x
Subject(s) - thylakoid , membrane , spinach , differential centrifugation , photosystem i , centrifugation , chloroplast , chemistry , chromatography , biophysics , photosystem ii , cytochrome , biochemistry , photosystem , biology , photosynthesis , enzyme , gene
The detergent Tween‐20 solubilized preferentially portions of the marginal regions of Spinacea oleracea L. thylakoid membranes and, thus, opened the inside of the grana to the external media. Differential centrifugation. following Tween‐20 solubilization. enabled separate fractions of grana and stromal‐exposed membranes to be isolated. Analysis of Tween‐20 solubilized material, after pelleting all membrane material by centrifugation at 100 000 g, revealed polypeptides associated with the coupling factor (CF 1 ) particles, cytochrome b 6 /f and photosystem II complexes, suggesting that the marginal membranes contain these proteins. Concomitantly, the 100 000 g pellet was depleted in cytochrome b 6 /f and P700, determined spectroscopically, Thus. our results reveal the margin to be a distinct membrane region, which does not contain the light‐harvesting centers of photosystem II (LHC II). The implication of these results, in terms of the energetic interaction of components of granal and stromalexposed membrane regions, is discussed.