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Detection and identification of gibberellins in extracts of Begonia leaves by bioassay, radioimmunoassay and gas chromatography – mass spectrometry
Author(s) -
Odén Per Christer,
Heide Ola M.
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb05424.x
Subject(s) - gibberellin , chromatography , bioassay , mass spectrometry , radioimmunoassay , chemistry , high performance liquid chromatography , gas chromatography , begonia , gas chromatography–mass spectrometry , botany , biology , biochemistry , genetics
Gibberellins A 1 (GA 1 ), GA 4 , GA 9 , GA 19 , and GA 20 were identified in extracts of leaves of Begonia x cheimantha Everett cv. Nova (Christmas or Lorraine Begonia). GA‐like substances were purified by reverse phase and normal phase high performance liquid chromatography (HPLC) and detected by Tan‐ginbozu dwarf rice bioassay and binding to antibodies raised against GA 1 , GA 4 and GA 9 . The final identifications were made by gas chromatography—mass spectrometry (GC‐MS).