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Fusicoccin, an inhibitor of brassinosteroid‐induced ethylene production
Author(s) -
Arteca Richard N.,
Bachman Jeannette M.,
Tsai DeSheng,
Mandava N. Bushan
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb02028.x
Subject(s) - fusicoccin , ethylene , vigna , brassinosteroid , chemistry , brassinolide , radiata , atp synthase , biochemistry , etiolation , biology , horticulture , enzyme , mutant , atpase , plant growth , arabidopsis , gene , catalysis
Fusicoccin was evaluated for its effects on brassinosteroid (BR), indole‐3‐acetic acid (IAA) and BR + IAA‐induced ethylene, 1‐aminocyclopropane‐1‐carboxylic acid (ACC) and ACC‐synthase production by etiolated mung bean ( Vigna radiata L. Rwilez cv. Berken) hypocotyl segments. Fusicoccin inhibition of ethylene and ACC production induced by 2 μ M BR started at concentrations as low as 0.05 μ M . Maximum inhibition occurred at a 1 μ M concentration with no further inhibition at higher concentrations tested. Fusicoccin (1 μ M ) was effective in the inhibition of BR‐induced ethylene, ACC and ACC‐synthase production at low and high concentrations of BR. Fusicoccin at concentrations as high as 2 μ M had no effect on ethylene and ACC production promoted by low concentrations of IAA (1 to 10 μ M ). When higher concentrations (100–1000 μ M ) of IAA were used, fusicoccin (1 μ M ) had an inhibitory effect on ethylene and ACC production. Interestingly, fusicoccin (1 μ M ) had little or no effect on ACC‐synthase promoted by high concentrations of IAA (1000 μ M ). When BR and IAA were used in combination, fusicoccin inhibited ethylene and ACC production at concentrations as low as 0.05 μ M with maximum inhibition occurring at 0.5 μ M . At a 1 μ M concentration, fusicoccin was effective in inhibiting the synergistic stimulation of ACC‐synthase promoted by BR and IAA.