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Photoautotrophic growth in suspension culture of cells from the moss, Barbula unguiculata
Author(s) -
Takami Shinji,
Nishikawa Shinji,
Takio Susumu,
Hino Seiichi
Publication year - 1988
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1988.tb00590.x
Subject(s) - dcmu , photosynthesis , biology , botany , moss , dry weight , sugar , chlorophyll , suspension culture , cell culture , food science , photosystem ii , genetics
Chlorophyllous cells in suspension culture from the moss, Barbula unguculata Hedw., grown under photoheterotrophic conditions were transferred to photoautotrophic conditions. The cells started to grow photoautotrophically without selection. Maximum growth was observed under irradiances of more than 5 W m 2 and in an atmosphere enriched with 1% (v/v) CO 2 . Under optimum growth conditions, dry weight and chlorophyll content in the culture had increased 20‐fold after 20 days of cell growth. High concentration of chlorophyll [10–20 μg (mg dry weight) −1 ] and high photosynthetic actively [30–70 μmol O 2 evolved (mg chlorophyll) −1 h −1 ] were observed throughout the culture period. In sugar‐free culture medium, cell growth did not occur in the dark or in the presence of 3‐(3,4‐dichlorophenyl)‐1,1‐dimethylurea (DCMU) under light, although cell growth was observed in glucose‐containing medium under those conditions. When cells that were grown photoautotrophically for one year were transferred to glucose‐containing medium under ordinary air, they started to grow heterotrophically both in the light and in the dark.