Premium
Ca 2+ and peroxidase derived from cultured peanut cells
Author(s) -
Hu Chunfang,
Lee David,
Chibbar Ravindra N.,
Huystee Robert B.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb08703.x
Subject(s) - peroxidase , horseradish peroxidase , egta , arachis hypogaea , chemistry , ionophore , chelation , biochemistry , incubation , cationic polymerization , nuclear chemistry , enzyme , calcium , biology , organic chemistry , botany , membrane
A 5% increase of Ca 2+ content of the incubation medium for cultured peanut ( Arachis hypogaea L.) cells caused a rise of peroxidase (EC 1.11.1.7) activity in the medium, which could be abolished by the addition of the chelator EGTA [eth‐yleneglycol‐bis‐(β‐aminoethyl ether)‐N,N,N',N'‐tetraacetic acid]. However, the determination of in vivo peroxidase synthesis in these cells showed that Ca 2+ had a direct effect on the biosynthesis rather than on transport alone. This concept was re‐enforced by the lack of effect by the ionophore A23187 on the transport. The Ca 2+ content was 2 and 5 mol (mol protein −1 ) for the cationic and anionic peanut peroxidase, respectively. The latter is different from the value reported for the anionic peroxidase from horseradish.