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Ribulose‐l,5‐bisphosphate carboxylase and photosystem II activities during chloroplast development in mesophyll and bundle sheath cells of Zea mays
Author(s) -
Caers Marleen,
Verbelen JeanPierre,
Vendrig Jan C.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb08698.x
Subject(s) - chloroplast , photosystem , vascular bundle , photosystem i , photosystem ii , photosynthesis , zea mays , biology , rubisco , pyruvate carboxylase , botany , chemistry , biochemistry , enzyme , gene , agronomy
Photosystem II (PS II) activity and the localization of ribulose‐l,5‐bisphosphate (RuBP) carboxylase (EC 4.1.1.39) were studied in primary leaves of young maize plants ( Zea mays L. cv. Fronica) by tetra‐nitro‐blue‐tetrazoliumchloride reduction and immunolocalization, respectively. In tissue of 3‐day‐old plants all chloroplasts were structurally identical. From day 4 they developed into their typical appearance of mesophyll and bundle sheath chloroplasts. First PS II‐activity was present in both types of chloroplasts. From day 4 it disappeared in bundle sheath chloroplasts concomitant with the loss of grana. RuBP carboxylase on the other hand was only present in bundle sheath chloroplasts at all stages of development. Thus, the control of the development of the photosystems and the Calvin cycle enzymes seem to differ.