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Immobilization of plant protoplasts on solid supports
Author(s) -
Vaňková Radomíra,
Bornman Chris H.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb08688.x
Subject(s) - protoplast , polylysine , bovine serum albumin , concanavalin a , sephadex , brassica , chromatography , chemistry , biochemistry , botany , biology , enzyme , in vitro
Brassica napus L. and Rosa sp. protoplasts were immobilized on variously treated Biosilon, Cytodex 1, 2 and 3, Separon, Sephadex, Sepharose and Sorfix micro carriers. Best anchorage of protoplasts of both species was obtained with Biosilon and Cytodex 3 coated with polylysine of high molecular weight (55 and 450 kDa), as well as with concanavalin A‐coated Cytodex 1. Precoating of micro carriers with bovine serum albumin was generally without effect.