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Preliminary analysis of root exudates of in vitro‐micropropagated Alnus glutinosa clones
Author(s) -
Vergnaud L.,
Chaboud A.,
Rougler M.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb06150.x
Subject(s) - biology , mucilage , alnus glutinosa , botany , dry weight , frankia , sugar , isoelectric focusing , symbiosis , horticulture , food science , root nodule , biochemistry , alder , bacteria , enzyme , genetics
Biochemical analysis at Alnus glutinosa (L.) Gaertn. root exudates was carried out in order to investigate the probable role of these exudates in the establishment of the Alnus‐Frankia symbiosis. Sufficient amounts of sterile root exudates were collected from plants originating from five in vitro‐micropropagated A. glutinosa clones (AG1, AG2, AG3, AG4, AG8) grown under aerobic conditions in nutrient solution with Ballotini glass beads. Cytological controls showed good preservation of roots at the end of the collecting period. The total protein and sugar contents of crude root exudates were determined and related to the dry root weight. These results showed a provenance effect. Soluble high molecular‐weight fractions (i.e. the mucilage) were isolated following rigorous purification of the crude root exudates and within this fraction proteins were characterized by isoelectric focusing. The protein pattern of the five clones tested showed major bands in common and some minor specific ones. These experimental data will serve as a useful basis for future physiological investigations.