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Nitrogenase is restricted to the vesicles in Frankia strain EAN1pec
Author(s) -
Meesters Titia M.,
Vliet Wihna M.,
Akkermans Antoon D. L.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb06143.x
Subject(s) - frankia , nitrogenase , hypha , immunogold labelling , vesicle , biology , strain (injury) , nitrogen fixation , electron microscope , microbiology and biotechnology , actinorhizal plant , biophysics , chemistry , biochemistry , botany , root nodule , ultrastructure , bacteria , anatomy , membrane , genetics , physics , optics
The presence of nitrogenase in vesicles and hyphae of Frankia EAN1pec was investigated by using immunogold labelling on ultrathin cryosections for electron microscopy. These studies resulted in the specific labelling of nitrogenase in the vesicles of nitrogen‐fixing cultures. No significant label could be found in the hyphae, indicating a strong repression of nitrogenase in the hyphae.