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Formation and regeneration of Frankia protoplasts
Author(s) -
Normand Philippe,
Simonet Pascal,
Prin Yves,
Moiroud André
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb06142.x
Subject(s) - frankia , protoplast , hypha , biology , regeneration (biology) , plasmid , microbiology and biotechnology , botany , spore , symbiosis , bacteria , gene , biochemistry , genetics , root nodule
A technique for forming protoplasts from Frankia cells and regenerating them to the normal hyphal mode of growth is described. Electron microscopy proved that protoplasts were studied and not spores or small hyphae. Regenerated colonies were investigated for genetic markers. One ArI3 colony had been cured of its plasmids without being affected in its symbiotic properties.