Premium
Identification and subgrouping of Frankia strains using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis
Author(s) -
Gardes M.,
Lalonde M.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb06138.x
Subject(s) - frankia , gel electrophoresis , polyacrylamide gel electrophoresis , biology , microbiology and biotechnology , sodium dodecyl sulfate , strain (injury) , electrophoresis , bacteria , botany , biochemistry , root nodule , symbiosis , enzyme , genetics , anatomy
Polypeptide patterns of soluble proteins from 35 Frankia strains from different plants of various geographical origins, belonging to Alnus and Elaeagnus host‐specificity groups were determined by one‐dimensional sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The polypeptide pattern was qualitatively the same for each strain whatever the number of subcultures or the age. Two gel electrophoresis groups A and E were observed which matched with the Alnus and Elaeagnus host‐specificity groups, but with some exceptions. The polypeptide patterns of the 35 Frankia strains tested were separated into 13 gel electrophoresis subgroups. Five Frankia strains were inoculated separately or in 3 mixed combinations of 2 strains on Alnus glutinosa (L.) Gaertn. plants. The polypeptide patterns of the re‐isolates obtained from 5‐month‐old nodules were identical to the corresponding strains used initially in the inoculum. Dual infection was observed on single plantlets.