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An abbreviated method for the isolation of mitochondria from storage tissues of oil seedlings
Author(s) -
Luster Douglas G.,
Fites Roger C.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb04315.x
Subject(s) - ricinus , cucumis , glycine , biology , sucrose , botany , storage protein , horticulture , chemistry , biochemistry , amino acid , gene
High yields of metabolically active mitochondria can be rapidly isolated from reserve storage tissues of castor bean (Ricinus communis L.), cucumber (Cucumis sativus L., cv. Early Green Cluster), cotton (Gossypium hirsutum L., cv. Coker 711), and soybean (Glycine max L., cvs Forrest and Centennial) seedlings by application of crude homogenates to discontinuous sucrose gradients, allowing for a rapid separation of organelles from detrimental factors present in cell homogenates.