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Activity of glyceraldehyde‐3‐phosphate dehydrogenase isozymes during photoperiodic floral induction in spinach leaves
Author(s) -
Bonzon Marc,
Simon Patrice,
Agosti Robert Degli,
Greppin Hubert,
Wagner Edgar
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb04308.x
Subject(s) - spinacia , isozyme , biology , glyceraldehyde 3 phosphate dehydrogenase , spinach , dehydrogenase , chloroplast , chenopodiaceae , flower induction , photoperiodism , cytosol , acclimatization , glyceraldehyde , biochemistry , botany , enzyme , gene
The activity of NAD + and NADP + ‐dependent glyceraldehyde‐3‐phosphate dehydrogenase isozymes (EC 1.2.1.12 and EC 1.2.1.13, respectively) were measured in spinach ( Spinacia oleracea L. cv. Nobel) leaves grown under different photoperiodic treatments in order to discriminate between the early events of floral induction and processes related to acclimation. Glycolysis‐linked isozyme activities were increased not only during floral induction and acclimation, but also during acclimation alone, suggesting that the changes in cytosolic activities were most probably associated with acclimation. In contrast, the chloroplast‐linked isozyme activities only increased during flower induction and appeared to be specifically associated with the initiation of the flowering process. The relative activity changes in the chloroplast and cytosol compartments may thus be supposed to be among the first signs of translation of the photoperiodic signal into cytosolic and cellular metabolic adaptation, whereby the leaf moves rapidly into a new metabolic state.