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Effects of hypochlorite disinfection on the response of barley aleurone layers to gibberellic acid
Author(s) -
Goudey J. Stephen,
Tittle Forrest L.,
Spencer Mary S.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb04290.x
Subject(s) - aleurone , hypochlorite , gibberellic acid , hordeum vulgare , chlorine , incubation , chemistry , sodium hypochlorite , botany , biology , poaceae , biochemistry , germination , enzyme , inorganic chemistry , organic chemistry
Although the use of hypochlorite to disinfect seeds is widespread, the effects on tissues isolated from them have been largely ignored. Disinfection of barley ( Hordeum vulgare L. cv. Himalaya) half‐seeds with hypochlorite solutions of ≥1.0% (w/v) available chlorine caused the pericarp to separate from the underlying tissues. Aleurone layers isolated from these grains had lower rates of oxygen consumption and released significantly less protein, PO 4 3− Mg 2+ , K + and amylase (EC 3.2.1.1) into the medium in response to gibberellic acid (GA 3 ) than layers isolated from grains disinfected with a 0.1% hypochlorite solution. Disinfection with 1.0% hypochlorite also quantitatively altered the spectrum of proteins released into the incubation medium by the layers in response to GA 3 .