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Nitrate‐induced de novo synthesis and regulation of NAD(P)H nitrate reductase from Sphagnum
Author(s) -
Deising Holger,
Rudolph Hansjörg
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb02887.x
Subject(s) - nitrate reductase , cycloheximide , in vivo , nitrate , sphagnum , spinacia , chemistry , biochemistry , nad+ kinase , in vitro , phytochrome , enzyme , biology , botany , protein biosynthesis , ecology , red light , microbiology and biotechnology , organic chemistry , chloroplast , gene , peat
The NO − 3 ‐triggered induction of nitrate reductase (NR; EC 1.6.6.2) in the bryophyte Sphagnum magellanicum Brid. has been studied, using in vivo and in vitro assays as well as immunological methods. The time‐course of induction was triphasic with maximal NR activity after 6–8 h. Results obtained from Western blots show that NR is synthesized de novo after NO − 3 application. The inhibitory effect of cycloheximide on NR induction corroborated this conclusion. Light enhanced the NO − 3 ‐triggered NR induction. The enzyme activity, measured in vivo, increased more than the in vitro activity. No evidence for phytochrome control of NR was found. Nitrate uptake, in contrast to NR activity, showed no lag period after NO − 3 application and, under the experimental conditions used, was not rate limiting for NR induction. Neither light nor a NO − 3 pretreatment significantly affected NO − 3 uptake.