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Effects of nitrogen source on betacyanin accumulation and growth in suspension cultures of Phytolacca americana
Author(s) -
Sakuta Masaaki,
Takagi Tsutomu,
Komamine Atsushi
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb02884.x
Subject(s) - ammonium , suspension culture , nitrate , nitrogen , suspension (topology) , chemistry , botany , biology , food science , cell culture , organic chemistry , genetics , mathematics , homotopy , pure mathematics
In suspension cultures of Phytolacca americana L., betacyanin accumulation per cell increased with increasing total nitrogen concentration (initial NH + 4 :NO − 3 ratio 1:2) in the range 0–40 m M and then remained almost constant in the range 40–80 m M . Increasing ammonium increased growth while betacyanin accumulation was reduced. On the other hand, betacyanin accumulation increased when nitrate was increased while growth was almost constant in the concentration range examined. A time‐course study of ammonium and nitrate concentration changes in the medium showed that betacyanin accumulation was associated with nitrate uptake.