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Heat inactivation of electron transport reactions in photosystem II particles
Author(s) -
Inoué Hiroshi,
Kitamura Tohru,
Noguchi Munenori
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb02881.x
Subject(s) - spinacia , photosystem ii , spinach , chemistry , divalent , electron transport chain , photosynthetic reaction centre , photochemistry , tricine , glutaraldehyde , nuclear chemistry , electron transfer , photosynthesis , chromatography , chloroplast , biochemistry , organic chemistry , gene
Heat inactivation of diphenylcarbazide (DPC)‐supported 2,6‐dichloroindophenol (DCIP) photoreduction by photosystem II (PS II) particles and non‐oxygen‐evolving PS II core complex isolated from spinach ( Spinacia oleracea L. cv. Kyoho) was suppressed under annealing conditions, and accelerated in the presence of EDTA or high concentration of divalent cations. After heating at 45°C for 10 min, half‐maximal annealing effects occurred at 35°C. Minimum acceleration was observed in the presence of 1 m M Mg 2+ , implying the existence of a cation‐specific site in the vicinity of the PS II reaction center. The acceleration depended on the temperature at which EDTA was added to PS II particles. Half‐acceleration by EDTA occurred at 35°C. Glutaraldehyde stabilized PS II particles against heat inactivation of PS II photochemical reactions.