Premium
Effect of an endogenous factor on arginase activity in germinating Bengal gram ( Cicer arietinum ) seeds
Author(s) -
Pawashe Aruna B.,
Srivastava S. K.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb02873.x
Subject(s) - arginase , germination , biology , biochemistry , in vivo , cotyledon , botany , arginine , amino acid , genetics
Arginase (EC 3.5.3.1) activity increased in the cotyledon, while it declined rapidly in the embryonic axis of Bengal gram ( Cicer arietinum L. cv. Desi type) seeds during germination. The decrease in enzyme activity in the embryonic axis was accompanied by changes in the properties of arginase in vitro such as decreased stability, increased heat lability and failure to bind to DEAE‐cellulose. These alterations were due to the presence of a low‐molecular‐weight factor in the extract, which was purified and identified as a hexose derivative. During germination the concentration of the factor increased in the embryonic axis, while no detectable level of the factor was present in the cotyledons. We postulate that the factor may have a role in the regulation of arginase activity in vivo.