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Abscisic acid in dormant apple seed tissues ‐ a rapid purification scheme using pre‐packed columns and GCMS‐SIM quantitation
Author(s) -
Subbaiah Thilak,
Powell Loyd
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb02868.x
Subject(s) - abscisic acid , endosperm , chromatography , malus , germination , chemistry , biology , botany , biochemistry , gene
A rapid small scale procedure has been developed for quantitative abscisic acid (ABA) determination by gas chromatography ‐ mass spectrometry with selected ion monitoping (GCMS‐SIM). Extracts of apple seeds ( Malus domestica cv. Northern Spy) were passed first through 3 ml C 18 columns, and then through 3 ml silica gel columns. GCMS‐SIM quantitation of ABA was done by adding an internal standard, hexadeuterated ABA. Ten sample extracts could be purified and analyzed by GCMS in 5 to 6 h, offering a quick and precise method to laboratories equipped with GC‐MS instrumentation. The endosperm membrane and seed coat of apple seeds contained 2.5‐3 times the ABA concentration found in the embryonic axis and cotyledons, supporting the hypothesis that it may be the ABA content of the membrane and seed coat that explains their inhibitory qualities with respect to seed germination.