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Nickel and rubidium uptake by whole oat plants in solution culture
Author(s) -
Aschmann S. G.,
Zasoski R. J.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb02866.x
Subject(s) - avena , rubidium , chemistry , transpiration , nickel , dinitrophenol , nuclear chemistry , dry weight , potassium , anaerobic exercise , zoology , botany , biochemistry , biology , photosynthesis , physiology , organic chemistry
Nickel and rubidium uptake by oat plants ( Avena sativa L. cv. Victory) were examined in relation to solution temperature, solution concentrations, metabolic inhibitors, anaerobic root conditions, transpiration and time. Over a 4‐h period, uptake rates for both Ni 2+ and Rb + remained constant at 23°C. Decreasing temperatures to 2°C, 20 μ M concentrations of 2,4‐dinitrophenol (DNP), or anaerobic root conditions decreased Ni 2+ and Rb + uptake rates by 97 to 86% in whole plants. Treatment of excised roots with 20 μ M DNP decreased Ni 2+ uptake by 93%. Nickel and Rb + uptake rates measured as a function of the external solution concentration followed a typical parabolic curve. K m (0.012 m M ) and V max [2.72 μmol (g dry weight) ‐1 h ‐1 ] values for Ni 2+ were nearly 7 times lower than those for Rb + [0.09 m M and 19.2 μmol (g dry weight) ‐1 h ‐1 ]. In all experiments, Ni 2+ and Rb + showed qualitatively similar uptake patterns, but Rb + uptake was quantitatively more sensitive than Ni 2+ to experimental manipulations.