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The role of ethylene in the regeneration of Helianthus annuus (sunflower) plants from callus
Author(s) -
Robinson Karol E. Paterson,
Adams Douglas O.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb02860.x
Subject(s) - hypocotyl , helianthus annuus , callus , seedling , sunflower , ethylene , helianthus , horticulture , explant culture , botany , regeneration (biology) , biology , etiolation , chemistry , in vitro , biochemistry , enzyme , microbiology and biotechnology , catalysis
Hypocotyl‐derived callus from the Helianthus annuus L. inbred line SS415B regenerated significantly more plants if the seedlings were grown in the light. The difference between light‐ and dark‐grown seedlings was not correlated with differences in seedling ethylene production, but seemed to be due to a difference in sensitivity to ethylene at a specific time during seedling growth. Treating 3‐day‐old dark‐grown seedlings with 10 μ M aminoethoxyvinylglycine (AVG) effectively inhibited ethylene production for at least 7 days. Hypocotyl callus derived from AVG‐treated seedlings gave the same amount of regeneration as callus from light‐grown seedlings. Promotion of regeneration by AVG was not seen unless the 3‐day‐old seedlings were grown for 4 additional days prior to culturing hypocotyl explants. The effects of AVG could be reversed by treatment with 1‐aminocyclopropane‐1‐carboxylic acid (ACC) during these 4 days. After the 4 days, ACC was no longer effective.