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Isolation and characterization of the enzymes involved in disintegration of the cell wall of Chlorella fusca
Author(s) -
Walter J. K.,
Aach H. G.
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb02847.x
Subject(s) - sporangium , enzyme , isoelectric point , biochemistry , cell wall , chlorella , chemistry , strain (injury) , biology , botany , algae , spore , anatomy
Using different chromatographical methods we have isolated an enzyme from the sporangia of Chlorella fusca Shihira et Krauss var. vacuolata , strain SAG 211–8b, which is responsible for the partial disintegration of the sporangium wall. We refer to it as carbohydrate‐releasing activity (CRA). It is an endoenzyme and splits oligosaccharides from the inner layer of the cell wall. In appropriate tests it shows β‐ d ‐fucosidase activity (EC 3.2.1.,38): The protein has a molecular weight around 45 kDa and an isoelectric point of pH 4.3; maximum activity is found at pH 5.4 and 60°C, although this temperature inactivates the enzyme quickly. β‐ d ‐Mannosidase (EC 3.2.1.25) and β‐ d ‐glucosidase (EC 3.2.1.21) were also found in the presence of CRA. These glycosidases were identified as exoenzymes. They are involved in the further degradation of the liberated obligosaccharides.