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Root primordia and endogenous auxin in maize roots cultured in vitro
Author(s) -
Golaz Franck,
Pilet PaulEmile
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb02833.x
Subject(s) - primordium , auxin , in vitro , endogeny , zea mays , biology , acetic acid , lateral root , botany , chemistry , biochemistry , agronomy , arabidopsis , gene , mutant
Quantitative analyses of indol‐3yl‐acetic acid (I aa ) in Zea mays L. (cv. LG 11) root segments cultured in vitro were performed by gas chromatography‐mass spectrometry with selected ion monitoring. The root extracts were first purified by highperformance liquid chromatography. Root primordia initiation in intact and decapitated roots showed different patterns: decapitation strongly enhanced primordia initiation in their first 10 mm. During the culture (5 days), I aa content decreased in both intact and decapitated roots. No correlation was found between the level of endogenous auxin and the numher of root primordia initiated from either intact or decapitated maize root segments.