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Isolation of root hairs from seedlings of Pisum sativum . Identification of root hair specific proteins by in situ labeling
Author(s) -
Röhm Mechthild,
Werner Dietrich
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb01955.x
Subject(s) - pisum , sativum , root hair , biology , incubation , botany , root tip , in situ , horticulture , chemistry , biochemistry , organic chemistry , gene
A procedure was developed which allows the large‐scale isolation of root hairs from seedlings of Pisum sativum . L. cvs. Kleine Rheinländerin and Rosa Krone. The method may yield up to 50 g fresh weight of root hairs per 3.10 4 seedlings. In a modified form considerable amounts of root hair material may be harvested, even after incubation of the roots in aqueous solutions. Thus, detailed biochemical studies on the root hair system have become feasible. The occurrence of specific proteins in membrane fractions of P. sativum root hairs was demonstrated as follows: Incubation of root hairs in situ with 3‐azidonaphthalene‐2,7‐disulfonate – a strongly anionic, photoactivated fluorescent marker – followed by gel electrophoresis of membrane fractions showed the presence of root‐hair specific proteins which, since the system was intact, suggests that they are on the outer surface of the cells.