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Isolation and characterisation of developing chloroplasts from light‐grown barley leaves
Author(s) -
GuillotSalomon Thérèse,
Farineau Nicole,
Cantrel Catherine,
Oursel Annette,
Tuquet Christiane
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb01953.x
Subject(s) - chloroplast , plastid , hordeum vulgare , biology , photosynthesis , photosystem ii , phosphatidylglycerol , photosystem i , chlorophyll , organelle , percoll , ultrastructure , botany , thylakoid , biochemistry , phospholipid , poaceae , centrifugation , membrane , phosphatidylcholine , gene
Developing chloroplasts were isolated from the basal region of green barley ( Hordeum vulgare L. cv. Menuet) leaves and their ultrastructure and biochemical composition were compared to those of mature chloroplasts from the tip of the same leaves, using two methods of purification on sucrose and Percoll gradients. When examined and compared to mature chloroplasts, the developing chloroplasts showed well‐developed grana stacks, but these last organelles were 2‐fold smaller and contained lower amounts of chlorohylls and polar lipids. Only traces of trans ‐3‐hexadecenoic acid could be detected in phosphatidylglycerol of developing plastids. The protein content of these plastids was higher than in mature plastids and showed an increased proportion of polypeptides linked to P‐700 chlorophyll α‐protein. The photosynthetic activity of these plastids was about 2‐fold lower and their photosystem 1/photosystem II ratio higher than in mature chloroplasts.