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Wheat endosperm mRNA and polysomes and their in vitro translation products during development and early stages of germination
Author(s) -
TercéLaforgue Thérèse,
Sallantin Marc,
Pernollet JeanClaude
Publication year - 1987
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1987.tb01952.x
Subject(s) - endosperm , polysome , gliadin , anthesis , caryopsis , germination , storage protein , protein biosynthesis , biology , messenger rna , rna , translation (biology) , in vitro , microbiology and biotechnology , biochemistry , botany , gene , ribosome , cultivar , gluten
We have examined the synthesis of the gliadins (alcohol‐soluble wheat storage proteins) from Triticum aestivum L. cv. Cappelle Desprez as a function of development from 2 days after anthesis until the beginning of seed germination with respect to free and membrane‐bound polysomes, cytoplasmic poly(A) + RNA and their in vitro translation products. The presence of gliadin mRNAs was investigated in mature dry seed and at the early stages of germination in relation to the lifetime of these mRNAs. Only one maximum, occurring at 18/21 days after anthesis, for mRNAs and polysomes as well as for their synthetic capacity, was observed. Nevertheless, some discrete proteins were expressed at different times during endosperm development, synthesized on either free or membrane‐bound polysomes. All gliadins were continuously synthesized from the earliest stages to maturity. Some long‐lived gliadin mRNAs carried over through the quiescent stage of the grain and were finally found in the germinating seed. From these observations, we conclude that the regulation of gliadin synthesis occurs not only at the transcriptional level but is also partly controlled by the activity of the protein synthesis machinery.