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Putative role of ethylene in Datura metel microspore embryogenesis
Author(s) -
Babbar Shashi B.,
Gupta Shrish C.
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb06609.x
Subject(s) - microspore , stamen , methionine , ethylene , silver nitrate , cobalt , chemistry , botany , datura , embryo , auxin , embryogenesis , biology , biochemistry , nuclear chemistry , organic chemistry , microbiology and biotechnology , amino acid , gene , pollen , catalysis
The role of ethylene in microspore embryogenesis was analyzed by studying the effect of cobalt ions, silver ions, methionine and Ethrel on the androgenic response of in vitro cultured anthers of Datura metel L. Cobalt and silver ions, provided in the form of cobalt chloride and silver nitrate, respectively, decreased the average number of embryos/plantlets developed per anther, at all the concentrations tested. In contrast, methionine (10 −5 –10 −3 M ) and Ethrel (10 −6 and 10 −5 M ) stimulated embryo formation. The optimal enhancement was recorded at 10 −5 M of both methionine and Ethrel.