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Potassium ion uptake by swelling Commelina communis guard cell protoplasts
Author(s) -
Fitzsimons Peter J.,
Weyers Jonathan D. B.
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb05953.x
Subject(s) - protoplast , guard cell , turgor pressure , biophysics , potassium , botany , ion transporter , swelling , chemistry , commelinaceae , plant cell , biology , biochemistry , membrane , materials science , organic chemistry , gene , composite material
Commelina communis L. guard cell protoplasts were induced to swell under low CO 2 conditions in the light while incubated in media containing KCl. Precise measurements of changes in the volume of the protoplasts were made including estimates of protoplast non‐osmotic volume by Boyle‐van't Hoff analysis. The amount of K + which accumulated during the treatment was measured. The observed changes in osmotic volume could be accounted for by the uptake of K + which appeared to be balanced by an anion or anions with an effective mean charge of – 1.63. The K + flux rates occurring in guard cell protoplasts were sufficient to explain guard cell turgor regulation in vivo.