Premium
Metabolism of benzyladenine by excised embryos of Picea abies
Author(s) -
Staden J.,
Forsyth C.,
Bergman L.,
Arnold S.
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb05946.x
Subject(s) - picea abies , embryo , cytokinin , metabolite , nucleotide , metabolism , biology , botany , distilled water , chemistry , biochemistry , chromatography , genetics , auxin , gene
Isolated embryos of Picea abies (L.) Karst. were pulsed with either distilled water, cold benzyladenine (BA) or [8‐ 14 C]‐BA for 3 h under sterile conditions, whereafter they were transferred to cytokinin‐free media for a further 24 h. Embryos of each treatment were then analysed for cytokinin activity. The embryos themselves yielded very little cytokinin activity. Analysis of the [ 14 C]‐BA‐treated material revealed that some residual BA was present even after 27 h of treatment but that the total radioactivity recovered from the embryos at this time was less than 10% of that recovered after the 3 h treatment. Apart from BA, the embryos contained other radioactive peaks, the major ones apparently being of a nucleotide nature. The BA‐containing medium in which the embryos were incubated initially was also analysed and again, apart from residual BA, the major metabolite(s) appeared to be BA‐nucleotide. However, two radioactive peaks were found in the medium which were absent from the embryos themselves and chemical tests indicated that these could be 7‐ and 9‐glucosides of BA. As the major metabolite resulting from the BA‐pulse was of a nucleotide nature and was found to be biologically active, it is postulated that the action of the BA may be mediated via the nucleotide.