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Polyethylene glycol and electric field treatment of plant protoplasts: characterization of some membrane properties
Author(s) -
HahnHägerdal Bärbel,
Hosono Kuniaki,
Zaehrisson Anders,
Bornman Chris H.
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb05748.x
Subject(s) - polyethylene glycol , electric field , protoplast , peg ratio , membrane , differential scanning calorimetry , biophysics , materials science , contact angle , chemistry , chemical engineering , biochemistry , biology , composite material , quantum mechanics , economics , thermodynamics , physics , finance , engineering
Petiolar protoplasts of a dihaploid line of winter oilseed rape Brassica napus L. ssp. oleifera were exposed to fusogenic polyethylene glycol (PEG) and electric field treatments. The surface properties and stability of membrane components of the treated protoplasts were investigated by contact angle measurements in aqueous two‐phase systems and differential scanning calorimetry, respectively. The leakage of intracellular components was estimated with respect to amino acids, proteins and DNA. Both fusogenic treatments resulted in the same apparent changes in membrane surface hydrophobicity and the same destabilization of membrane components. However, the PEG‐treated protoplasts were more leaky than both the control and the electric field‐treated protoplasts. The results indicate that the molecular mechanisms of PEG‐ and electrical field‐induced fusion are similar. However, the effects of the latter appear to be less harmful presumably because the parameters for electric field treatment are more easily controlled.