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The effect of abscisic acid on epidermal cells: protoplast swelling and ATPase activity
Author(s) -
Itai Chanan,
RothBejerano Nurit
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb05596.x
Subject(s) - abscisic acid , protoplast , atpase , swelling , stimulation , biochemistry , sodium , chemistry , biophysics , microsome , biology , microbiology and biotechnology , enzyme , endocrinology , organic chemistry , chemical engineering , engineering , gene
Isolated epidermal protoplasts of Commelina communis L. increase in volume in the presence of KCl. Since this swelling is an osmotic phenomenon it reflects K + influx. ATP slightly decreased the volume of the protoplasts, pointing towards the possibility that K + uptake is passive. On the other hand abscisic acid (ABA) and sodium orthovanadate increased the swelling, and their effect was reversed by ATP. This may support the suggestion that ABA inhibits the active and ATPase‐mediated relase of K + from epidermal cells. Mg 2+ ‐dependent, K + ‐stimulated ATPase activity was found in the microsomal fraction from epidermal cells. This activity was vandadate sensitive. ABA increased the basal activity in the presence of Mg 2+ but inhibited the K + stimulation.