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Nucleotide pools in leaf and root tissue of tobacco plants: Influence of leaf senescence
Author(s) -
Meyer Reiner,
Wagner Karl G.
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb05075.x
Subject(s) - nucleotide , senescence , energy charge , nicotiana tabacum , nad+ kinase , nucleoside , biochemistry , adenine nucleotide , biology , chemistry , botany , enzyme , adenylate kinase , genetics , gene
The content of the different ribonucleotides, nucleoside diphosphate sugars, NAD + and NADP + was determined in the leaves and roots of single plants of Nicotiana tabacum L. cv. Samsun using a new procedure. The determination makes use of extraction with HClO 4 followed by extract purification and separation by a combination of anion‐exchange and reversed phase high performance liquid chromatography. The largest pools were the adenine nucleotides and the uracil nucleotides with nucleoside diphosphate sugars as the main fraction. Leaf senescence was accompanied by a strong reduction of the nucleotide content of the leaves, but the adenine nucleotide‐derived energy charge remained at a high level and even increased at the later stages of senescence. This indicates a balanced metabolism also in the older leaves and excludes the possibility that leaf senescence is triggered by the energy charge.