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Root influence on in vitro growth of hyphae of the mycorrhizal mushroom Cantharellus cibarius replaced by carbon dioxide
Author(s) -
Straatsma Gerben,
Griensven Leo J. L. D.,
Bruinsma Johan
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb05050.x
Subject(s) - hypha , mycelium , biology , botany , boletus , mushroom , carbon dioxide , mycorrhiza , fungus , horticulture , symbiosis , bacteria , genetics , ecology
The presence of a living root of tomato ( Lycopersicon esculentum Mill. cv. Panase F) had a strong positive effect on growth in vitro of hyphal fragments of Cantharellus cibarius Fr. This was observed for 12 out of 14 fungal strains tested. Initial re‐growth of hyphae was independent of the presence of a root, but for the further development of strong mycelial growth 7 strains completely depended on the presence of a root. Five strains showed a considerably prolonged lag phase in the absence of a root and only 2 strains were independent. The presence of a root had no influence on the specific growth rates. Hyphal fragments of the totally root‐dependent strain S1 grew strongly in the absence of a root if they were brought into intimate mutual contact. Factor(s) stimulating growth were apparently produced by both roots and fungus. Volatiles from donor cultures of tomato roots of C. cibarius, Boletus edulis , and Tylopilus felleus stimulated growth of hyphal fragments in root‐less receiver cultures. This effect was prevented by the presence of a KOH solution. Donor cultures could be replaced by 0.5% CO 2 .