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Free space uptake and influx of Ni 2+ in excised barley roots
Author(s) -
Körner Lena E.,
Møller Ian M.,
Jensén Paul
Publication year - 1986
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1986.tb03400.x
Subject(s) - hordeum vulgare , chemistry , dinitrophenol , fraction (chemistry) , nickel , biophysics , poaceae , nuclear chemistry , botany , chromatography , biochemistry , biology , organic chemistry
The free space uptake and influx of Ni 2+ ( 63 Ni 2+ ) was characterized in excised roots of 7‐day‐old, dark‐grown barley ( Hordeum vulgare L. cv. Møyar). Most of the Ni 2+ ( 63 Ni 2+ ) present in the roots after a typical experiment was removed by washing in CaCl 2+ , unlabelled NiCl 2 or EDTA solutions. The amount of loosely bound Ni 2+ increased during the first few minutes in the uptake solution, and then remained constant. A small fraction was tightly bound, probably mainly in the cell walls. Binding, both loose and tight, increased with concentration, as did influx. However, the lower the external Ni 2+ concentration, the larger was the proportion of Ni 2+ tightly bound compared with the total amount of Ni 2+ taken up. Ni 2+ influx was lowered by 85% with 2,4‐dinitrophenol, which may indicate that Ni 2+ is actively absorbed across the plasmalemma.